Toxin–antigen conjugates as selection tools for antibody producing cells
Copyright policy )Toxin–antigen conjugates as selection tools for antibody producing cells
The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure. Initial experiments were done with methotrexate as low molecular weight toxin and fluorescein as model antigen. Methotrexate and a methotrexate-fluorescein conjugate were characterized regarding their toxicity. Afterwards the effect of the fluorescein-specific antibody B13-DE1 on the toxicity of the methotrexate-fluorescein conjugate was determined. Finally, first results showed that hybridoma cells that produce fluorescein specific antibodies are able to grow in the presence of fluorescein-toxin-conjugates.
- University of Potsdam Germany
Mice, Inbred BALB C, Hybridomas, Dose-Response Relationship, Drug, Cell Survival, Ovalbumin, Immunotoxins, Antibodies, Monoclonal, Reproducibility of Results, Fluoresceins, Antibodies, Mice, Methotrexate, Immunologic Techniques, Animals, Antigens, Antibody-Producing Cells, Institut für Biochemie und Biologie
Mice, Inbred BALB C, Hybridomas, Dose-Response Relationship, Drug, Cell Survival, Ovalbumin, Immunotoxins, Antibodies, Monoclonal, Reproducibility of Results, Fluoresceins, Antibodies, Mice, Methotrexate, Immunologic Techniques, Animals, Antigens, Antibody-Producing Cells, Institut für Biochemie und Biologie
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