Soluble Dectin-1 as a tool to detect β-glucans

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2006 United States, United Kingdom English Publisher:Elsevier BVJournal:Journal of Immunological Methods, volume 314, pages 164-169 (issn: 0022-1759,

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Authors: Graham, Lisa; Tsoni, S. Vicky; Willment, Janet A.; Williams, David L.; Taylor, Philip R.; Gordon, Siamon; Dennehy, Kevin; +1 Authors

doi: 10.1016/j.jim.2006.05.013

pmid: 16844139

Soluble Dectin-1 as a tool to detect β-glucans

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Abstract

Beta-glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian beta-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

Countries

United States, United Kingdom

Related Organizations

University of Cape Town
South Africa
University of Oxford
United Kingdom
East Tennessee State University James H. Quillen College of Medicine
United States
East Tennessee State University
United States

Keywords

beta-glucan, 570, beta-Glucans, Recombinant Fusion Proteins, 610, Enzyme-Linked Immunosorbent Assay, Nerve Tissue Proteins, Saccharomyces cerevisiae, Cell Line, Fungal Proteins, Antibody Specificity, Cell Wall, Humans, Lectins, C-Type, innate immunity, Glucans, Membrane Proteins, Flow Cytometry, C-type lectin, Microscopy, Fluorescence, Solubility, ELISA, Surgery, dectin-1, Dectin-1

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