Cytotoxic effects of Cochlospermum regium (Mart &amp; Schrank) Pilger aqueous root extract on mammalian cells

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2006 English Publisher:Elsevier BVJournal:Journal of Ethnopharmacology, volume 103, pages 302-305 (issn: 0378-8741,

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Authors: Livônios, Ceschini; Elida Geralda, Campos;

doi: 10.1016/j.jep.2005.07.026

pmid: 16198078

Cytotoxic effects of Cochlospermum regium (Mart & Schrank) Pilger aqueous root extract on mammalian cells

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Abstract

We investigated the effect of Cochlospermum regium (Mart & Schrank) Pilger aqueous root extract on Chinese hamster ovarian (CHO)-K1 cells. The extract significantly decreased proliferation of CHO-K1 cells (EC(50)=1.5mg/mL). Apoptosis induction was analysed by fluorescent microscopy. Cell cultures treated with Cochlospermum regium extract for 4h contained 13.6% apoptotic cells after 24h (investigated by fluorescent DNA-microscopy with acridine orange/ethidium bromide staining). Characteristic chromatin condensation and fragmentation, verified by 4',6-diamidino-2-phenylindole (DAPI) staining, was observed in the cells after treatment with Cochlospermum regium extract. The results confirm the toxicity of Cochlospermum regium root extract to immortal, non-tumorigenic mammalian cells in vitro.

Related Organizations

University of Brasília
Brazil

Keywords

Cricetulus, Plant Extracts, Cricetinae, Animals, Bixaceae, Apoptosis, CHO Cells, Plant Roots

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