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Antibodies for CFTR studies

Authors: Mendes, Filipa; Farinha, Carlos; Roxo-Rosa, Mónica; Fanen, Pascale; Edelman, Aleksander; Dormer, Robert; Mcpherson, Margaret; +8 Authors

Antibodies for CFTR studies

Abstract

For most expression studies focusing on the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein, sensitive and specific antibodies (Abs) are critically needed. Several Abs have been produced commercially or by research laboratories for CFTR detection in both cell lines with heterologous or endogenous expression and native cells/tissues. Here, we review the applicability of most Abs currently in use in CF research for the biochemical and/or immunocytochemical detection of CFTR.

Country
France
Keywords

Pulmonary and Respiratory Medicine, 570, Cystic Fibrosis Transmembrane Conductance Regulator, Expression, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Anti-CFTR, Antibodies, Cell Line, 618, Monoclonal, Humans, Pediatrics, Perinatology, and Child Health, MESH: Cystic Fibrosis Transmembrane Conductance Regulator, MESH: Humans, MESH: Antibodies, Immunotechniques, Antibodies, Monoclonal, MESH: Cell Line, Immunologic Techniques, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, MESH: Immunologic Techniques, Western, Immunocytochemistry

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    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 10%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
33
Top 10%
Top 10%
Top 10%
hybrid