AbstractAn increase in ADP hydrolysis was observed in spinal cord synaptosomal fractions of 2‐month‐old Wistar male rats, when compared to other ages (1, 4 and 6 months of age), while no change in ATPase activity was observed. Conversely, in female rats, whilst no change in ADPase activity was observed in the spinal cord synaptosomal fraction, ATPase activity diminished with age, in 1–6‐month‐old animals. 5′‐Nucleotidase activity was higher in the 4‐month‐old male and female rats in relation to 1 and 2‐month‐old animals. In the female rats, this activity continued to increase at least until 6 months of age.In conclusion, adenine nucleotides hydrolysis in synaptosomes from rat spinal cord is influenced by age and by gender. Since both ATP and adenosine may act as neuromodulators in the spinal cord, influencing several processes such as nociception, the regulation of ATP‐metabolizing enzymes in spinal cord is probably important for the normal function of this tissue at different ages.