Abstract We have reported numerous clinical cases where a patient's unilateral chewing was thought to have reduced hearing ability. The purpose of this study is to determine whether occlusion and chewing function affect auditory evoked magnetic fields (AEFs). The subjects (seven males, 20–29) signed consent forms approved by the institutional ethical committee. A button sensor (FlexiForce™, Nitta) was used to monitor a subject's occlusal pressure. The subjects were instructed to maintain three ranges of their maximum occlusal pressure in each experiment. Subjects bit on cotton roll on the left or right first molar sites and were then stimulated with pure tone bursts (80 dB, 1000 Hz) from the left or right. The source localization was based on the signals recorded with the 36 planar gradiometers near both sides of the auditory area and the analysis was carried out based on the time-varying, multi-dipole model. The equipment used to monitor a subject's occlusal pressure did not recognize the noise affecting the AEF's measurement. The equivalent current dipoles (ECDs) measured at 20% or less than the maximum occlusal pressure, and between 20% and 40% of the maximum occlusal pressure confirmed existing in less than 1 mm by the MRI image as compared with control. This shows that the localization of ECDs exists in the auditory area at less than 40% of the maximum occlusal pressure. The AEF's response to a stimulus on the right shows a lower value compared with controls during cotton roll biting. Biting on cotton roll affected the AEF's response and the response was related to the strength of the occlusal force.