Notch 1 signaling pathway effect on implantation competency
Copyright policy )Notch 1 signaling pathway effect on implantation competency
To investigate the relationship between the Notch 1 signaling pathway and the embryo implantation rate.Mouse embryos were cultured in vitro, and implantation competency was quantified.Tertiary fertility center of a university teaching hospital.Outbred ICR strain mouse embryos.The expression of Notch 1 was altered by adding a γ-secretase inhibitor to the culture medium. We quantified the consequent effect on embryo implantation.We measured the messenger RNA level of Notch 1 gene at different embryonic stages, embryo implantation rate under different culture conditions, the amount of Notch 1, and related implantation competency.Quantitative polymerase chain reaction showed that the expression of Notch 1 increased during the implantation window. Adding γ-secretase inhibitor in the culture medium decreased the percentage of blastocysts in a dose-dependent manner. A Matrigel invasion assay showed that the competency of implantation required adequate expression of Notch 1 intracellular domain.Expression of Notch 1 at the proper time is required for the competency of embryo implantation; this effect is mediated through regulation of Notch 1 intracellular domain expression.
- National Defense Medical Center Taiwan
- Tri-Service General Hospital Taiwan
Mice, Inbred ICR, Time Factors, Dose-Response Relationship, Drug, Gene Expression Regulation, Developmental, Embryo, Mammalian, Real-Time Polymerase Chain Reaction, Embryo Culture Techniques, Drug Combinations, Mice, Animals, Female, Proteoglycans, Collagen, Embryo Implantation, Laminin, RNA, Messenger, Amyloid Precursor Protein Secretases, Enzyme Inhibitors, Receptor, Notch1, Signal Transduction
Mice, Inbred ICR, Time Factors, Dose-Response Relationship, Drug, Gene Expression Regulation, Developmental, Embryo, Mammalian, Real-Time Polymerase Chain Reaction, Embryo Culture Techniques, Drug Combinations, Mice, Animals, Female, Proteoglycans, Collagen, Embryo Implantation, Laminin, RNA, Messenger, Amyloid Precursor Protein Secretases, Enzyme Inhibitors, Receptor, Notch1, Signal Transduction
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