Loop-mediated isothermal amplification for the rapid detection of Salmonella
Copyright policy )Loop-mediated isothermal amplification for the rapid detection of Salmonella
Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay was found to be >2.2 cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay. S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8 cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for Salmonella.
DNA, Bacterial, Bacteriological Techniques, Base Sequence, Molecular Sequence Data, Salmonella enterica, Polymerase Chain Reaction, Sensitivity and Specificity, Salmonella, Food Microbiology, Serotyping, Nucleic Acid Amplification Techniques
DNA, Bacterial, Bacteriological Techniques, Base Sequence, Molecular Sequence Data, Salmonella enterica, Polymerase Chain Reaction, Sensitivity and Specificity, Salmonella, Food Microbiology, Serotyping, Nucleic Acid Amplification Techniques
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