Evidence for coordination of lysosomal (ASMase) and plasma membrane (NSMase2) forms of sphingomyelinase from mutant mice
Copyright policy )Evidence for coordination of lysosomal (ASMase) and plasma membrane (NSMase2) forms of sphingomyelinase from mutant mice
NSMase2 is associated to the plasma membrane, whereas ASMase is predominantly lysosomal; both hydrolyze sphingomyelin (SM) to ceramide and phosphocholine. Although SM accumulated in both ASMase−/− and fro/fro (NSMase2−/−) fibroblasts, the reduction of ceramides was more dramatic in fro/fro cells. ASMase mRNA, protein and enzyme activity were substantially elevated in fro/fro fibroblasts. In contrast, NSMase2 activity was unaffected in ASMase−/− fibroblasts. ASMase−/− cells showed normal cell cycling whereas fro/fro cells grew slowly and were arrested in G1/G0 and could be corrected by transfection with smpd3 gene. This suggests two distinct subcellular pathways for SM catabolism with distinct functions.
- University of Chicago United States
- University of Chicago United States
- University of Chicago United States
- University of Chicago United States
- THE UNIVERSITY OF CHICAGO United States
Male, Phosphorylcholine, Blotting, Western, Ceramides, Resting Phase, Cell Cycle, Mice, Animals, NSMase2, Cells, Cultured, Mice, Knockout, Mice, Inbred C3H, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Cell Membrane, G1 Phase, Cell Cycle Checkpoints, Fibroblasts, Flow Cytometry, Sphingomyelin Phosphodiesterase, Coordination, ASMase, Female, Lysosomes
Male, Phosphorylcholine, Blotting, Western, Ceramides, Resting Phase, Cell Cycle, Mice, Animals, NSMase2, Cells, Cultured, Mice, Knockout, Mice, Inbred C3H, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Cell Membrane, G1 Phase, Cell Cycle Checkpoints, Fibroblasts, Flow Cytometry, Sphingomyelin Phosphodiesterase, Coordination, ASMase, Female, Lysosomes
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