A Solanum tuberosum inositol phosphate kinase (StITPK1) displaying inositol phosphate–inositol phosphate and inositol phosphate–ADP phosphotransferase activities
Copyright policy )A Solanum tuberosum inositol phosphate kinase (StITPK1) displaying inositol phosphate–inositol phosphate and inositol phosphate–ADP phosphotransferase activities
We describe a multifunctional inositol polyphosphate kinase/phosphotransferase from Solanum tuberosum, StITPKα (GenBank accession number: EF362784), hereafter called StITPK1. StITPK1 displays inositol 3,4,5,6‐tetrakisphosphate 1‐kinase activity: K m = 27 μM, and V max = 19 nmol min−1 mg−1. The enzyme displays inositol 1,3,4,5,6‐pentakisphosphate 1‐phosphatase activity in the absence of a nucleotide acceptor and inositol 1,3,4,5,6‐pentakisphosphate–ADP phosphotransferase activity in the presence of physiological concentrations of ADP. Additionally, StITPK1 shows inositol phosphate‐inositol phosphate phosphotransferase activity. Homology modelling provides a structural rationale of the catalytic abilities of StITPK1. Inter‐substrate transfer of phosphate groups between inositol phosphates is an evolutionarily conserved function of enzymes of this class.
- National Institutes of Health United States
- National Institute of Health Pakistan
- Research Triangle Park Foundation United States
- National Institute of Environmental Health Sciences United States
- University of East Anglia United Kingdom
Inositol Phosphates, Molecular Sequence Data, Phosphotransferase, ATP-grasp fold, 540, Catalysis, Phosphoric Monoester Hydrolases, Protein Structure, Secondary, Inositol polyphosphate kinase, Substrate Specificity, Adenosine Diphosphate, Phosphotransferases (Alcohol Group Acceptor), 616, Amino Acid Sequence, Cloning, Molecular, Plant Proteins, Solanum tuberosum
Inositol Phosphates, Molecular Sequence Data, Phosphotransferase, ATP-grasp fold, 540, Catalysis, Phosphoric Monoester Hydrolases, Protein Structure, Secondary, Inositol polyphosphate kinase, Substrate Specificity, Adenosine Diphosphate, Phosphotransferases (Alcohol Group Acceptor), 616, Amino Acid Sequence, Cloning, Molecular, Plant Proteins, Solanum tuberosum
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