Recent studies have highlighted the importance of paracrine growth factors as mediators of pro‐angiogenic effects by endothelial progenitor cells (EPCs), but little is known about the release of lipid‐based factors like endocannabinoids by EPCs. In the current study, the release of the endocannabinoids anandamide and 2‐arachidonoylglycerol by distinct human EPC sub‐types was measured using HPLC/tandem mass‐spectrometry. Anandamide release was highest by adult blood colony‐forming EPCs at baseline and they also demonstrated increased 2‐arachidonoylglycerol release with TNF‐α stimulation. Treatment of mature endothelial cells with endocannabinoids significantly reduced the induction of the pro‐inflammatory adhesion molecule CD106 (VCAM‐1) by TNF‐α.