Egr‐1, a new downstream molecule of Epstein‐Barr virus latent membrane protein 1
Copyright policy )Egr‐1, a new downstream molecule of Epstein‐Barr virus latent membrane protein 1
To investigate the effect of Epstein‐Barr virus (EBV) latent membrane protein 1 (LMP1) on human cancer cells, we sought to identify and analyze potential target genes that were differentially expressed in the presence and absence of LMP1. Our cDNA microarray analysis revealed that expression of early growth response gene‐1 (Egr‐1) was increased by LMP1 expression in MCF7 and Jurkat cells. An NFκB inhibitor (SN50) antagonized LMP1‐induced enhancement of Egr‐1 expression, indicating that LMP1 induced Egr‐1 via NFκB. Furthermore, three lines of evidence indicated that Egr‐1 was required for LMP1‐induced cancer cell survival. First, Egr‐1 expression enhanced the survival of doxorubicin‐treated MCF7 cells. Second, inhibition of Egr‐1 expression by siRNA (siEgr‐1) effectively suppressed LMP‐1‐induced survival of MCF7 cells. Third, Egr‐1 knockdown decreased LMP1‐induced expression of Bfl‐1. Similar relationships among EBV infection, Egr‐1 and drug resistance were also observed in tissues of peripheral T‐cell lymphoma‐unspecified (PTCL‐u) patients.
- Gyeongsang National University Korea (Republic of)
- BIOMEDICAL RESEARCH INSTITUTE
- Biomedical Research Institute United States
- Department of Pathology Taiwan
- BIOMEDICAL RESEARCH INSTITUTE
Egr-1, Epstein-Barr Virus Infections, Survival, Cell Survival, NF-kappa B, Antiviral Agents, Up-Regulation, Gene Expression Regulation, Neoplastic, Viral Matrix Proteins, Jurkat Cells, Drug Resistance, Viral, Epstein-Barr virus, Humans, RNA, Messenger, RNA, Small Interfering, LMP1, Herpesviridae, Early Growth Response Protein 1
Egr-1, Epstein-Barr Virus Infections, Survival, Cell Survival, NF-kappa B, Antiviral Agents, Up-Regulation, Gene Expression Regulation, Neoplastic, Viral Matrix Proteins, Jurkat Cells, Drug Resistance, Viral, Epstein-Barr virus, Humans, RNA, Messenger, RNA, Small Interfering, LMP1, Herpesviridae, Early Growth Response Protein 1
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