Nitrogenase Fe protein‐like Fe–S cluster is conserved in L‐protein (BchL) of dark‐operative protochlorophyllide reductase from Rhodobacter capsulatus
Copyright policy )Nitrogenase Fe protein‐like Fe–S cluster is conserved in L‐protein (BchL) of dark‐operative protochlorophyllide reductase from Rhodobacter capsulatus
Dark‐operative protochlorophyllide reductase (DPOR) in bacteriochlorophyll biosynthesis is a nitrogenase‐like enzyme consisting of L‐protein (BchL‐dimer) as a reductase component and NB‐protein (BchN–BchB‐heterotetramer) as a catalytic component. Metallocenters of DPOR have not been identified. Here we report that L‐protein has an oxygen‐sensitive [4Fe–4S] cluster similar to nitrogenase Fe protein. Purified L‐protein from Rhodobacter capsulatus showed absorption spectra and an electron paramagnetic resonance signal indicative of a [4Fe–4S] cluster. The activity quickly disappeared upon exposure to air with a half‐life of 20 s. These results suggest that the electron transfer mechanism is conserved in nitrogenase Fe protein and DPOR L‐protein.
- Nagoya University Japan
- Kanagawa University Japan
- Kanazawa University Japan
Iron-Sulfur Proteins, Oxidoreductases Acting on CH-CH Group Donors, Spectrum Analysis, Electron Spin Resonance Spectroscopy, Light-Harvesting Protein Complexes, Bacteriochlorophyll biosynthesis, Fe–S cluster, Rhodobacter capsulatus, Bacterial Proteins, Nitrogenase, Oxidoreductases, Protochlorophyllide reductase, Conserved Sequence, BchL
Iron-Sulfur Proteins, Oxidoreductases Acting on CH-CH Group Donors, Spectrum Analysis, Electron Spin Resonance Spectroscopy, Light-Harvesting Protein Complexes, Bacteriochlorophyll biosynthesis, Fe–S cluster, Rhodobacter capsulatus, Bacterial Proteins, Nitrogenase, Oxidoreductases, Protochlorophyllide reductase, Conserved Sequence, BchL
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).58 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!