Herpetomonas spp. isolated from tomato fruits (Lycopersicon esculentum) in southern Spain

descriptionPublicationkeyboard_double_arrow_right Article 01 May 2007 France English Publisher:Elsevier BVJournal:Experimental Parasitology, volume 116, pages 88-90 (issn: 0014-4894,

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Authors: Marin, Clotilde; Fabre, Sandrine; Sanchez Moreno, Manuel; Dollet, Michel;

doi: 10.1016/j.exppara.2006.11.003

pmid: 17266953

Herpetomonas spp. isolated from tomato fruits (Lycopersicon esculentum) in southern Spain

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Abstract

A flagellate of the family Trypanosomatidae was isolated from fruits of Lycopersicon esculentum (tomato) in southeastern Spain. The isolate was successfully adapted to in vitro culture in monophasic media. The morphology showed the kinetoplast to be positioned towards the middle of the body, and the typical opistomastigote form characteristic of members of the genus Herpetomonas. Amplification of the mini-exon gene was negative, whilst for the 5S ribosomal rRNA gene the result was positive. The DNA sequence was obtained and its alignment with other trypasomatids, obtained using the BLAST algorithm, suggested it was closely related to Herpetomonas samuelpessoai.

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France

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Keywords

RNA, Spliced Leader, Molecular Sequence Data, http://aims.fao.org/aos/agrovoc/c_6618, http://aims.fao.org/aos/agrovoc/c_49903, arn, Solanum lycopersicum, Sequence Homology, Nucleic Acid, Animals, H20 - Maladies des plantes, http://aims.fao.org/aos/agrovoc/c_4475, Plant Diseases, Base Sequence, http://aims.fao.org/aos/agrovoc/c_27583, RNA, Ribosomal, 5S, http://aims.fao.org/aos/agrovoc/c_26618, Exons, séquence nucléotidique, DNA, Protozoan, Phytomonas, morphologie, Spain, http://aims.fao.org/aos/agrovoc/c_7273, Trypanosomatina

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