Periphyton developments in water distribution canals induce major disturbances for system management, such as clogging problems when fixed algae are detached. Periphyton models can be used to simulate and improve canal management. The purpose of this paper is to review the periphyton models which integrate a hydrodynamic effect, and to discuss their relevance for application in open-channels. Afterwards, a new model of periphyton detachment that integrates the hydrodynamic factor is proposed. An experiment in semi-real conditions is performed to compare periphyton development under four different hydrodynamic regimes. The proposed model is compared on experimental results with two existing models. The new model reproduces well the periphyton dynamics in the four canals simultaneously.