Quantification of mitral regurgitation (MR) by echocardiography is integral to assessing lesion severity and entails the integration of multiple Doppler-based parameters. These methods are founded primarily upon the principle of proximal isovelocity surface area (PISA), a two-dimensional (2D) method known to involve several assumptions regarding MR jet characteristics. The authors analyzed the results of a semiautomated method of three-dimensional (3D)-based regurgitant volume (RVol) estimation that accounts for jet behavior throughout the cardiac cycle and compared it with conventional 2D PISA methods for MR quantification.A total of 50 patients referred for transesophageal echocardiography for evaluation of primary (n = 25) and secondary (n = 25) MR were included for analysis. Three-dimensional full-volume color data sets were acquired, along with standard 2D methods for PISA calculation. A 3D semiautomated MR flow quantification algorithm was applied offline to calculate 3D RVol, with simultaneous temporal curves generated from the 3D data set. Three-dimensional RVol was compared with 2D RVol. Three-dimensional vena contracta area was also performed in all cases.There was a modest correlation between 2D RVol and 3D RVol (r = 0.60). The semiautomated 3D approach resulted in significantly lower values of RVol compared with 2D PISA. Real-time and dynamic flow curve patterns were used for integral estimates of 3D RVol over the cardiac cycle, with a distinct bimodal pattern in functional MR and a brief and solitary peak in primary MR.Using a semiautomated 3D software for the quantification of MR allows the simultaneous calculation of 3D RVol with an automated generation of dynamic flow curves characteristic of the underlying MR mechanism. The present flow curve pattern results highlight well-known differences between MR flow dynamics in degenerative MR compared with functional MR.