Isolated mouse inner cell mass is unable to reconstruct trophectoderm

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2011 English Publisher:Elsevier BVJournal:Differentiation, volume 82, pages 1-8 (issn: 0301-4681,

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Authors: Katarzyna, Szczepanska; Lukasz, Stanczuk; Marek, Maleszewski;

doi: 10.1016/j.diff.2011.04.001

pmid: 21514715

Isolated mouse inner cell mass is unable to reconstruct trophectoderm

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Abstract

The ability of ICM to differentiate into TE is still a controversial issue. Many of authors have showed the reconstruction of TE from isolated ICMs. We showed that immunosurgical method is not 100% efficient and that the original TE cells very often remain on the surface of isolated ICMs. We also found that isolated ICM cells cultured in vitro do not express Cdx2, and that the TE is reconstituted from TE cells which have survived immunosurgery. This indicates that very soon after the formation of TE in the blastocyst, the cells of ICM lose the potency to differentiate into trophectoderm.

Related Organizations

University of Warsaw
Poland

Keywords

Homeodomain Proteins, Microscopy, Confocal, Cell Differentiation, Immunohistochemistry, Trophoblasts, Mice, Blastocyst, Blastocyst Inner Cell Mass, Ectoderm, Animals, CDX2 Transcription Factor, Female, Cells, Cultured

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