Diseases of the central nervous system (CNS) are relatively common in food animals. Potential causes include infectious agents, nutritional deficiencies, metabolic disorders, genetic defects, toxins, and idiopathic causes. Determining the correct etiologic diagnosis often depends on a thorough postmortem examination and collection of samples. This article reviews some of the steps and procedures necessary to collect the necessary information on CNS diseases in food animals. Techniques for the examination of the CNS are briefly described, and some of the gross pathology likely to be encountered in a food animal practice is reviewed.