Recently, a commercial product has been made available (equine follicle-stimulating hormone [eFSH]) for superovulating mares. This has provided the practitioner with a hormonal product that is readily available for enhancing multiple ovulations. Additional benefits of stimulating multiple follicles include an increased number of follicles available for oocyte collection, availability of extra embryos for embryo freezing, enhancement of fertility in subfertile mares, and advancement of the first ovulation of the year. This article provides a short historical background, but most of it centers on the use of eFSH for stimulation of follicular development and ovulation in mares.