Vetiveria zizanioides L. Nash (Family: Poaceae) root (intact and spent) extracts and fractions were evaluated for antimycobacterial activity against Mycobacterium tuberculosis H(37)Rv and H(37)Ra strains using radiometric BACTEC 460 TB system.The ethanolic extract of intact as well as spent root were showed potent antituberculosis activity at a minimum concentration of 500μg/mL. The hexane fraction also showed antibacterial action by recording continuous decline in growth index (GI) of M. tuberculosis at 50μg/mL. It was furthermore observed that root extract and hexane fraction showed activity even after the extraction of essential oil by hydro-distillation.Our results suggest that ethanolic extract and hexane fraction exhibited potent antimycobacterial activity.The observed potential inhibitory characteristic not only supports the traditional medicinal uses of vetiver but also signify a promising candidature of root extract and hexane fraction of V. zizanioides as an antituberculosis agent.