Fat grafting to the face for volume augmentation, and skin rejuvenation have become a popular procedure. However, the main obstacles to fat grafting include the unpredictable volume maintenance rate and the unpredictable number of treatments needed to obtain a satisfactory rejuvenate effect. Therefore, many patients need repeat sessions. However, serial fat grafting with fresh fat imposes a burden on the patient not only because of the pain but also because of the downtime of harvesting. Therefore, if the fat can be cryopreserved, and used many times in 1 harvesting, those burdens can be reduced.