Molecular analysis of lungs from pigs immunized with a mutant transferrin binding protein B-based vaccine and challenged with Haemophilus parasuis
Copyright policy )Molecular analysis of lungs from pigs immunized with a mutant transferrin binding protein B-based vaccine and challenged with Haemophilus parasuis
The molecular analysis of pigs vaccinated with a mutant transferrin-binding protein B (Y167A) from Haemophilus parasuis was compared with that performed for unvaccinated challenged (UNCH) and unvaccinated unchallenged (UNUN) pigs. Microarray analysis revealed that UNCH group showed the most distinct expression profile for immune response genes, mainly for those genes involved in inflammation or immune cell trafficking. This fact was confirmed by real-time PCR, in which the greatest level of differential expression from this group were CD14, CD163, IL-8 and IL-12. In Y167A group, overexpressed genes included MAP3K8, CD14, IL-12 and CD163. Proteomics revealed that collagen α-1 and peroxiredoxins 2 and 6 were overexpressed in Y167A pigs. Our study reveals new data on genes and proteins involved in H. parasuis infection and several candidates of resistance to infection that are induced by Y167A vaccine. The expression of proinflammatory molecules from Y176A pigs is similar to their expression in UNUN pigs.
- University of Córdoba Spain
- University of Córdoba (Spain) Spain
- University of Leon Spain
- Universidade de Passo Fundo Brazil
Inflammation, Proteomics, Swine Diseases, Sanidad animal, Swine, Glässer’s disease, Ganado porcino, Microarray, Real-Time Polymerase Chain Reaction, Mass Spectrometry, Transferrin-Binding Protein B, Haemophilus parasuis, Bacterial Proteins, Tissue Array Analysis, Bacterial Vaccines, Mutation, Vaccines, Subunit, Animals, Cytokines, Immunization, Subunit Vaccines, Lung
Inflammation, Proteomics, Swine Diseases, Sanidad animal, Swine, Glässer’s disease, Ganado porcino, Microarray, Real-Time Polymerase Chain Reaction, Mass Spectrometry, Transferrin-Binding Protein B, Haemophilus parasuis, Bacterial Proteins, Tissue Array Analysis, Bacterial Vaccines, Mutation, Vaccines, Subunit, Animals, Cytokines, Immunization, Subunit Vaccines, Lung
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).9 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!