Purification and sensitivity of Clostridium chauvoei hemolysin to various erythrocytes

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2006 English Publisher:Elsevier BVJournal:Comparative Immunology, Microbiology and Infectious Diseases, volume 29, pages 263-268 (issn: 0147-9571,

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Authors: Bernard, Mudenda Hang'ombe; Tomoko, Kohda; Masafumi, Mukamoto; Shunji, Kozaki;

doi: 10.1016/j.cimid.2006.06.002

pmid: 16914203

Purification and sensitivity of Clostridium chauvoei hemolysin to various erythrocytes

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Abstract

Using ammonium sulphate fractionation, the Clostridium chauvoei hemolysin was purified by cation exchange chromatography and sephacryl S-100 gel filtration. The molecular mass of the hemolysin, determined by SDS-PAGE was found to be approximately 27kDa. The activity of the hemolysin was determined in erythrocytes of various animals, with sensitivities observed in the order of cow, sheep, chicken, rabbit, rat, mouse, dog and horse. Temperature affected the sensitivity of erythrocytes to C. chauvoei hemolysin. These results may reflect distinct characteristics of the hemolytic activity of C. chauvoei hemolysin and that the hemolysin may be pore-forming.

Related Organizations

University of Zambia
Zambia
Osaka Metropolitan University
Japan

Keywords

Molecular Weight, Hemolysin Proteins, Erythrocytes, Chromatography, Gel, Animals, Clostridium chauvoei, Electrophoresis, Polyacrylamide Gel, Chromatography, Ion Exchange, Sensitivity and Specificity

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