Reduction of 4-nitrophenol catalyzed by nitroreductase

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2007 English Publisher:Elsevier BVJournal:Chinese Chemical Letters, volume 18, pages 10-12 (issn: 1001-8417,

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Authors: Jie Chen; Rong Ji Dai; Bin Tong; Sheng Yuan Xiao; Weiwei Meng;

doi: 10.1016/j.cclet.2006.11.009

Reduction of 4-nitrophenol catalyzed by nitroreductase

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Abstract

Abstract The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper. 4-Amino-phenol and 4-hydroxylamino-phenol were found in the reductive products. The relationship between reaction time and the reductive ratio were studied. The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions. The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygen-insensitive enzyme. The reductive products of 4-nitrophenol were determined by HPLC and MS.

Related Organizations

Beijing Institute of Technology
China (People's Republic of)
Beijing University of Chinese Medicine
China (People's Republic of)

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