Tetrabromobisphenol A (TBBPA) is currently the most widely-used brominated flame retardant (BFR) and has been proven to have a very high toxicity to aquatic organisms including bivalves. In this study, a laboratory experiment was conducted to obtain a better understanding of the role of algae food on the bioaccumulation of TBBPA and its effects on the suspension-feeding bivalve, the scallop, Chlamys farreri. Scallops were exposed to TBBPA via algae food alone or food+water for 10 days. Results showed that TBBPA was accumulated rapidly by scallops, reaching an approximate steady state in soft tissues within 3 days. The primary route of TBBPA accumulation was via the water, while dietary uptake was relatively minor. TBBPA exposure led to significant inhibition on microsomal cytochrome P450 and cytochrome b5 levels in gills and digestive gland, whereas GST activity and GSH level increased significantly, which indicated that TBBPA could be a suitable substrate to directly participate in phase II metabolism. TBBPA clearly induced the activity of SOD, suggesting the oxidant stress induced by TBBPA. This study suggests that dietary uptake was not the predominant uptake route for TBBPA in bivalves and also provides a preliminary basis for studying the detoxification and antioxidant responses of marine bivalves upon exposure to TBBPA.