descriptionPublicationkeyboard_double_arrow_right Article 01 May 2018 English Publisher:Elsevier BVJournal:Carbohydrate Research, volume 460, pages 47-50 (issn: 0008-6215,

Authors: Andrei V. Perepelov; Sof'ya N. Senchenkova; Olesya I. Naumenko; Bin Yang; Alexander S. Shashkov; Wenqi Hou; Yuriy A. Knirel; +1 Authors

doi: 10.1016/j.carres.2018.02.008

pmid: 29524726

Structure and genetics of a glycerol 2-phosphate-containing O-specific polysaccharide of Escherichia coli O33

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Abstract

An O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O33 followed by gel-permeation chromatography on Sephadex G-50. The polysaccharide was found to contain glycerol 2-phosphate (Gro-2-P), and the following structure of its tetrasaccharide repeat was established by sugar analysis, dephosphorylation, and 1D and 2D 1H and 13C NMR spectroscopy: The O33-antigen gene cluster was analyzed and found to be essentially consistent with the O-polysaccharide structure.

Related Organizations

Department of Chemistry and Material Sciences
Russian Federation
University of Technology
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Russian Academy of Sciences
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Nankai University
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Tianjin Medical University
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Keywords

Lipopolysaccharides, Multigene Family, Polysaccharides, Bacterial, Escherichia coli, O Antigens

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