The gut microbiota of humans is complex but stable in composition and function. Metabolic conversions performed by the members of the microbiota yield both beneficial and hazardous compounds, and have a systematic impact on human health. Comparative studies have shown that the microbiota of patients, suffering from a number of diseases, is in dysbiosis, which is characterized by a distinct composition. Compositional differences have also been noted between members of geographically distant healthy populations. To be able to identify which compositional changes promote compromised health, it is of interest to identify members of the microbiota that perform essential metabolic transformations. This review provides an insight into the microbial contribution to the metabolism of carbohydrates, proteins and bile acids, and focuses on the link between diversity and function.