The reaction of intracellular Ca(2+) to different agonist stimuli in primary hepatocytes from rainbow trout (Oncorhynchus mykiss) as well as the permanent fish cell line RTL-W1 was investigated systematically. In addition to "classical" agonists such as phenylephrine and ATP, model environmental toxicants like 4-nitrophenol and 3,4-dichloroaniline were used to elucidate possible interactions between toxic effects and Ca(2+) signaling. We report Ca(2+) oscillations in response to several stimuli in RTL-W1 cells and to a lesser extent in primary hepatocytes. Moreover, these Ca(2+) oscillations are amplitude-encoded in contrast to their mammalian counterpart. Bioinformatics and computational analysis were employed to identify key players of Ca(2+) signaling in fish and to determine likely causes for the experimentally observed differences between the Ca(2+) dynamics in fish cells compared to those in mammalian liver cells.