The condition for pseudo-first-order kinetics in enzymatic reactions is independent of the initial enzyme concentration

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 2004 English Publisher:Elsevier BVJournal:Biophysical Chemistry, volume 107, pages 165-174 (issn: 0301-4622,

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Authors: S, Schnell; C, Mendoza;

doi: 10.1016/j.bpc.2003.09.003

pmid: 14962597

The condition for pseudo-first-order kinetics in enzymatic reactions is independent of the initial enzyme concentration

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Abstract

The linearization of the Michaelis-Menten reaction by pseudo-first-order kinetics is revised. A phase-plane analysis allows the derivation of a new condition for its validity that is directly linked to the reaction efficiency, and contrary to widely established knowledge, is independent of the initial enzyme concentration.

Related Organizations

Instituto Venezolano de Investigaciones Científicas
Venezuela

Keywords

Kinetics, Numerical Analysis, Computer-Assisted, Enzymes

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