A new route to dTDP-6-deoxy-l-talose and dTDP-l-rhamnose: dTDP-l-rhamnose 4-epimerase in Burkholderia thailandensis

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2011 English Publisher:Elsevier BVJournal:Bioorganic & Medicinal Chemistry Letters, volume 21, pages 3,914-3,917 (issn: 0960-894X,

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Authors: Hye-Gyeong, Yoo; So-Yeon, Kwon; Suman, Karki; Hyung-Jin, Kwon;

doi: 10.1016/j.bmcl.2011.05.030

pmid: 21640586

A new route to dTDP-6-deoxy-l-talose and dTDP-l-rhamnose: dTDP-l-rhamnose 4-epimerase in Burkholderia thailandensis

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Abstract

dTDP-L-rhamnose (dTDP-Rha)-synthesizing dTDP-6-deoxy-L-lyxo-4-hexulose reductase (4-KR) and dTDP-Rha 4-epimerase were characterized from Burkholderia thailandensis E264 by utilizing rmlD(Bth) (BTH_I1472) and wbiB(Bth) (BTH_I1476), respectively. Incubation of the recombinant WbiB(Bth) with RmlA/RmlB/RmlC/Tal, which has previously been shown to generate dTDP-6-deoxy-L-talose (dTDP-6dTal) from α-D-glucose-1-phosphate, dTTP, and NADPH, produced dTDP-Rha. (1)H NMR measurements confirmed that both RmlA/RmlB/RmlC/Tal/WbiB(Bth) and RmlA/RmlB/RmlC/RmlD produced dTDP-Rha. WbiB(Bth) alone produced dTDP-Rha when incubated with dTDP-6dTal. This is the first report to demonstrate epimerase activity interconverting between dTDP-Rha and dTDP-6dTal.

Related Organizations

Myongji University
Korea (Republic of)

Keywords

Molecular Structure, Burkholderia, Nucleoside Diphosphate Sugars, Deoxy Sugars, Thymine Nucleotides, Carbohydrate Epimerases, Chromatography, High Pressure Liquid, Recombinant Proteins, Hexoses

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