Eyebrow reconstruction is a challenge because eyebrow hair is unique. Although various surgical methods have been used to reconstruct eyebrow defects, to the best of our knowledge, the use of eyebrow free flaps for eyebrow reconstruction has not been reported. We describe the successful reconstruction of a large eyebrow defect using a supraorbital artery perforator free flap and show that microvascular eyebrow transplantation is another tool for reconstructing large eyebrow defects. Accordingly, we recommend that microvascular eyebrow transplantation be considered a useful option for the reconstruction of large eyebrow defects.