Organophosphorus hydrolase multilayer modified microcantilevers for organophosphorus detection
Copyright policy )Organophosphorus hydrolase multilayer modified microcantilevers for organophosphorus detection
We report a biosensor based on organophosphorus hydrolase (OPH) multilayer modified microcantilever (MCL) for detection of organophosphorus compounds (OPs). The assay is based on substrate-dependent bending of the OPH functionalized MCLs. The cantilever bending amplitude at equilibrium was a function of the concentration of paraoxon with the dynamic range extending from 10(-7) to 10(-3)M. The lower detection limit of approximately 10(-7)M for paraoxon was an order of magnitude better than the OPH-based potentiometric and optical biosensors based on pH modulation. There was a good intra-sensor and an acceptable inter-sensor reproducibility as evidenced by the standard errors of 5% and 15%, respectively. OPs measured using this technique included parathion and diisopropyl fluorophosphate (DFP) in the order of paraoxon>DFP>parathion. The conformational change of the OPH was most likely the main origin of MCL bending.
- Louisiana Tech University United States
- University of California, Riverside United States
Aryldialkylphosphatase, Transducers, Reproducibility of Results, Biosensing Techniques, Equipment Design, Mechanics, Sensitivity and Specificity, Elasticity, Equipment Failure Analysis, Photometry, Organophosphorus Compounds, Coated Materials, Biocompatible, Stress, Mechanical
Aryldialkylphosphatase, Transducers, Reproducibility of Results, Biosensing Techniques, Equipment Design, Mechanics, Sensitivity and Specificity, Elasticity, Equipment Failure Analysis, Photometry, Organophosphorus Compounds, Coated Materials, Biocompatible, Stress, Mechanical
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