PLP catabolism: Identification of the 4-pyridoxic acid dehydrogenase gene in Mesorhizobium loti MAFF303099

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 2007 English Publisher:Elsevier BVJournal:Bioorganic Chemistry, volume 35, pages 458-464 (issn: 0045-2068,

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Authors: Tathagata, Mukherjee; Cynthia, Kinsland; Tadhg P, Begley;

doi: 10.1016/j.bioorg.2007.07.003

pmid: 17868768

pmc: PMC2140245

PLP catabolism: Identification of the 4-pyridoxic acid dehydrogenase gene in Mesorhizobium loti MAFF303099

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Abstract

The function of the mlr6793 gene from Mesorhizobium loti MAFF303099 has been identified. This gene encodes 4-pyridoxic acid dehydrogenase, an enzyme involved in the catabolism of PLP (Vitamin B6). This enzyme was overexpressed in Escherichia coli and characterized. 4-Pyridoxic acid dehydrogenase is a 33kDa protein that catalyzes the four electron oxidation of 4-pyridoxic acid to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate, using nicotinamide adenine dinucleotide as a cofactor. The k cat for NADH production is 0.01s(-1). The KM values for 4-pyridoxic acid and NAD are 5.8 and 6.6microM, respectively.

Related Organizations

Cornell University
United States

Keywords

Alcohol Oxidoreductases, Rhizobiaceae, Escherichia coli, Pyridoxine, Cloning, Molecular, Chromatography, High Pressure Liquid

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