Protein engineering of formate dehydrogenase
Copyright policy )Protein engineering of formate dehydrogenase
NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is one of the best enzymes for the purpose of NADH regeneration in dehydrogenase-based synthesis of optically active compounds. Low operational stability and high production cost of native FDHs limit their application in commercial production of chiral compounds. The review summarizes the results on engineering of bacterial and yeast FDHs aimed at improving their chemical and thermal stability, catalytic activity, switch in coenzyme specificity from NAD+ to NADP+ and overexpression in Escherichia coli cells.
- A N Bach Institute of Biochemistry Russian Federation
- Lomonosov Moscow State University Russian Federation
- Department of Biological Sciences Russian Federation
- Russian Academy of Sciences Russian Federation
Models, Molecular, Sequence Homology, Amino Acid, Molecular Sequence Data, Coenzymes, Protein Engineering, Formate Dehydrogenases, Recombinant Proteins, Bioreactors, Yeasts, Mutation, Escherichia coli, Amino Acid Sequence
Models, Molecular, Sequence Homology, Amino Acid, Molecular Sequence Data, Coenzymes, Protein Engineering, Formate Dehydrogenases, Recombinant Proteins, Bioreactors, Yeasts, Mutation, Escherichia coli, Amino Acid Sequence
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