Novel protein–protein interactions between Entamoeba histolytica d-phosphoglycerate dehydrogenase and phosphoserine aminotransferase
Copyright policy )Novel protein–protein interactions between Entamoeba histolytica d-phosphoglycerate dehydrogenase and phosphoserine aminotransferase
Physical interactions between d-phosphoglycerate dehydrogenase (EhPGDH) and phosphoserine aminotransferase (EhPSAT) from an enteric human parasite Entamoeba histolytica was observed by pull-down assay, gel filtration chromatography, chemical cross-linking, emission anisotropy, molecular docking and molecular dynamic simulations. The protein-protein complex had a 1:1 stochiometry with a dissociation constant of 3.453 × 10(-7) M. Ionic interactions play a significant role in complex formation and stability. Analysis of the energy minimized average simulated model of the protein complex show that the nucleotide binding domain of EhPGDH specifically interacts with EhPSAT. Denaturation studies suggest that the nucleotide binding domain (Nbd) and substrate binding domain (Sbd) of EhPGDH are independent folding/unfolding units. Thus the Nbd-EhPGDH was separately cloned over-expressed and purified to homogeneity. Fluorescence anisotropy study show that the purified Nbd interacts with EhPSAT. Forward enzyme catalyzed reaction for the EhPGDH-PSAT complex showed efficient Km values for 3-phosphoglyceric acid as compared to only EhPGDH suggesting a possibility of substrate channelling in the protein complex.
Protein Folding, Binding Sites, Protein Conformation, Entamoeba histolytica, Molecular Dynamics Simulation, Catalysis, Recombinant Proteins, Substrate Specificity, Structure-Activity Relationship, Humans, Protein Interaction Domains and Motifs, Phosphoglycerate Dehydrogenase, Transaminases, Protein Binding
Protein Folding, Binding Sites, Protein Conformation, Entamoeba histolytica, Molecular Dynamics Simulation, Catalysis, Recombinant Proteins, Substrate Specificity, Structure-Activity Relationship, Humans, Protein Interaction Domains and Motifs, Phosphoglycerate Dehydrogenase, Transaminases, Protein Binding
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