Mcl-1 inhibits Mff-mediated mitochondrial fragmentation and apoptosis

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2020 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 523, pages 620-626 (issn: 0006-291X,

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Authors: Yangpei, Liu; Yunyun, Ma; Zhuang, Tu; Chenshuang, Zhang; Mengyan, Du; Yong, Wang; Fangfang, Yang; +2 Authors

doi: 10.1016/j.bbrc.2019.12.104

pmid: 31941601

Mcl-1 inhibits Mff-mediated mitochondrial fragmentation and apoptosis

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Abstract

Myeloid cell leukemia-1 (Mcl-1) is involved in the regulation of mitochondrial fission and fusion. This report aims to explore whether Mcl-1 can interact with mitochondrial fission factor (Mff) and regulate Mff-mediated mitochondrial fragmentation and apoptosis. Fluorescence images of living cells coexpressing YFP-Mff and CFP-Mcl-1 showed that Mcl-1 markedly inhibited Mff-mediated mitochondrial fragmentation and apoptosis, suggesting that Mcl-1 played a key role in inhibiting mitochondrial fission. The cells coexpressing YFP-Mff and CFP-Mcl-1 exhibited consistent fluorescence resonance energy transfer (FRET) efficiency with that of the cells coexpressing CFP-Mcl-1 and YFP, demonstrating that Mcl-1 did not directly bind to Mff on mitochondria. Collectively, Mcl-1 inhibits Mff-mediated mitochondrial fission and apoptosis not via directly binding to Mff on mitochondria.

Related Organizations

Jinan University
China (People's Republic of)
University of Jinan
China (People's Republic of)
South China Normal University
China (People's Republic of)

Keywords

Mitochondrial Proteins, Humans, Membrane Proteins, Myeloid Cell Leukemia Sequence 1 Protein, Apoptosis, Mitochondrial Dynamics, HeLa Cells, Mitochondria

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