Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis
Copyright policy )Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis
The nicotinamidase/pyrazinamidase PncA is a member of a large family of hydrolase enzymes that catalyze the deamination of nicotinamide to nicotinic acid. PncA also functions as a pyrazinamidase in a wide variety of eubacteria and is an essential coenzyme in many cellular redox reactions in living systems. We report the crystal structure of substrate-free PncA from Bacillus subtilis (BsPncA) at 2.0 Å resolution to improve our understanding of the PncA family. The structure of BsPncA consists of an α/β domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the α/β domain, while the subdomain is highly flexible. Both dimers and tetramers were observed in BsPncA protein crystals, but only dimers were observed in solution. Our results provide useful information that will further enhance our understanding of the molecular functions of PncA family members.
- Ministry of Education of the People's Republic of China China (People's Republic of)
- Korea University Korea (Republic of)
- Seoul National University Korea (Republic of)
- Dalian Minzu University China (People's Republic of)
- Minzu University of China China (People's Republic of)
Bacterial Proteins, Protein Domains, Protein Conformation, Protein Multimerization, Crystallography, X-Ray, Amidohydrolases, Bacillus subtilis, Nicotinamidase
Bacterial Proteins, Protein Domains, Protein Conformation, Protein Multimerization, Crystallography, X-Ray, Amidohydrolases, Bacillus subtilis, Nicotinamidase
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