Enhanced ceramide-induced apoptosis in ceramide kinase overexpressing cells

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2007 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 354, pages 309-314 (issn: 0006-291X,

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Authors: Christine, Graf; Philipp, Rovina; Loïc, Tauzin; Andrea, Schanzer; Frédéric, Bornancin;

doi: 10.1016/j.bbrc.2006.12.208

pmid: 17222802

Enhanced ceramide-induced apoptosis in ceramide kinase overexpressing cells

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Abstract

We evaluated how increased levels of ceramide kinase (CerK) would impact the growth of COS-1 fibroblasts and RBL-2H3 basophils. The low CerK activity in these cells was strongly up-regulated upon recombinant expression of CerK. CerK-overexpressing COS-1 cells depended on higher concentrations of serum for their growth and displayed many filipodia. The two CerK-overexpressing cell lines were more sensitive to C2-ceramide-mediated apoptosis, and this correlated with the production of C2-ceramide-1-phosphate by CerK. This study indicates that ceramide kinase may participate in the control of cell growth, and establishes a novel assay that will be valuable for testing ceramide kinase inhibitors.

Related Organizations

Novartis (Switzerland)
Switzerland
Novartis (Austria)
Austria

Keywords

Phosphotransferases (Alcohol Group Acceptor), Dose-Response Relationship, Drug, COS Cells, Chlorocebus aethiops, Animals, Apoptosis, Ceramides

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