Regulation of cholesterol 25-hydroxylase expression by vitamin D3 metabolites in human prostate stromal cells
Copyright policy )Regulation of cholesterol 25-hydroxylase expression by vitamin D3 metabolites in human prostate stromal cells
Vitamin D3 plays an important role in the control of cell proliferation and differentiation. Cholesterol 25-hydroxylase (CH25H) is an enzyme converting cholesterol into 25-hydroxycholesterol. Vitamin D3 as well as 25-hydroxycholesterol has been shown to inhibit cell growth and induce cell apoptosis. Here we show that 10 nM 1alpha,25(OH)2D3 and 500 nM 25OHD3 upregulate CH25H mRNA expression in human primary prostate stromal cells (P29SN). Protein synthesis inhibitor cycloheximide does not block 1alpha,25(OH)2D3 mediated upregulation of CH25H mRNA. Transcription inhibitor actinomycin D blocks basal level as well as 1alpha,25(OH)2D3 induced CH25H mRNA expression. 1alpha,25(OH)2D3 has no effect on CH25H mRNA stability. 25-Hydroxycholesterol significantly decreased the P29SN cell number. A CH25H enzyme inhibitor, desmosterol, increases basal cell number but has no significant effect on vitamin D3 treated cells. Our data suggest that ch25h could be a vitamin D3 target gene and may partly mediate anti-proliferative action of vitamin D3 in human primary prostate stromal cells.
- Tampere University Hospital Finland
- University of Turku Finland
- Tampere University Finland
Male, Protein Synthesis Inhibitors, Time Factors, Dose-Response Relationship, Drug, Desmosterol, Prostate, Apoptosis, Gene Expression Regulation, Steroid Hydroxylases, Dactinomycin, Humans, RNA, Messenger, Cycloheximide, Stromal Cells, Cells, Cultured, Cell Proliferation, Cholecalciferol
Male, Protein Synthesis Inhibitors, Time Factors, Dose-Response Relationship, Drug, Desmosterol, Prostate, Apoptosis, Gene Expression Regulation, Steroid Hydroxylases, Dactinomycin, Humans, RNA, Messenger, Cycloheximide, Stromal Cells, Cells, Cultured, Cell Proliferation, Cholecalciferol
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).20 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!