Nuclease Rsn from Rhizopus stolonifer: specificity and mode of action
Copyright policy )Nuclease Rsn from Rhizopus stolonifer: specificity and mode of action
Nuclease Rsn from Rhizopus stolonifer catalyzes the hydrolysis of ss- and dsDNA in a ratio of approximately 2:1. Time course of 3' and 5' terminal analysis of the hydrolytic products of ss- and dsDNA showed that nuclease Rsn does not show any strict base preference and cleaves DNA in a non-specific manner. Moreover, separation of the hydrolytic products of ss- and dsDNA in the presence of Mg2+, Mn2+ or Co2+ showed the predominance of tetra-, tri-, and dinucleotides followed by mononucleotides, suggesting an endo mode of action.
Manganese, Buffaloes, Phosphoric Diester Hydrolases, Hydrolysis, Cobalt, DNA, Endonucleases, Substrate Specificity, Intestines, Liver, Animals, Cattle, Magnesium, Rhizopus, Spleen, Snake Venoms
Manganese, Buffaloes, Phosphoric Diester Hydrolases, Hydrolysis, Cobalt, DNA, Endonucleases, Substrate Specificity, Intestines, Liver, Animals, Cattle, Magnesium, Rhizopus, Spleen, Snake Venoms
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