Autoregulation of bone sialoprotein gene in pre-osteoblastic and non-osteoblastic cells

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 2004 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 316, pages 461-467 (issn: 0006-291X,

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Authors: Qisheng, Tu; Masato, Yamauchi; Steven C, Pageau; Jake Jinkun, Chen;

doi: 10.1016/j.bbrc.2004.02.068

pmid: 15020240

Autoregulation of bone sialoprotein gene in pre-osteoblastic and non-osteoblastic cells

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Abstract

Regulation of the bone sialoprotein (BSP) gene is important in the differentiation of osteoblasts, in bone matrix mineralization, and in tumor metastasis. We investigated BSP gene transcription by performing functional analysis of the 9256bp of the 5' flanking region of the murine BSP gene containing its promoter. We found that the forced expression of BSP stimulated mouse BSP promoter activity in a dose-dependent manner in both MC3T3-E1 preosteoblast and HEK-293 cell lines, which was transcriptional factor Cbfa1 independent. Co-culture of cells separately expressing BSP promoter reporter and BSP failed to mediate the BSP autoregulation, suggesting that the event might happen intracellularly. Deletion analysis of the BSP promoter indicated that the proximal promoter (110bp) was sufficient to confer this autoregulation. We conclude that the BSP gene is autoregulated in part by a positive feedback on its own promoter.

Related Organizations

Kanagawa Dental College
Japan
Tufts University
United States

Keywords

Transcriptional Activation, Osteoblasts, Sialoglycoproteins, Stem Cells, Genetic Vectors, Cytomegalovirus, Regulatory Sequences, Nucleic Acid, Coculture Techniques, Cell Line, Mice, Animals, Homeostasis, Humans, Integrin-Binding Sialoprotein, RNA, Messenger, Promoter Regions, Genetic

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