Crystal structures of a type-1 ribosome inactivating protein from Momordica balsamina in the bound and unbound states
Copyright policy )Crystal structures of a type-1 ribosome inactivating protein from Momordica balsamina in the bound and unbound states
The ribosome inactivating proteins (RIPs) of type 1 are plant toxins that eliminate adenine base selectively from the single stranded loop of rRNA. We report six crystal structures, type 1 RIP from Momordica balsamina (A), three in complexed states with ribose (B), guanine (C) and adenine (D) and two structures of MbRIP-1 when crystallized with adenosine triphosphate (ATP) (E) and 2'-deoxyadenosine triphosphate (2'-dATP) (F). These were determined at 1.67Å, 1.60Å, 2.20Å, 1.70Å, 2.07Å and 1.90Å resolutions respectively. The structures contained, (A) unbound protein molecule, (B) one protein molecule and one ribose sugar, (C) one protein molecule and one guanine base, (D) one protein molecule and one adenine base, (E) one protein molecule and one ATP-product adenine molecule and (F) one protein molecule and one 2'-dATP-product adenine molecule. Three distinct conformations of the side chain of Tyr70 were observed with (i) χ(1)=-66°and χ(2)=165° in structures (A) and (B); (ii) χ(1)=-95° and χ(2)=70° in structures (C), (D) and (E); and (iii) χ(1)=-163° and χ(2)=87° in structure (F). The conformation of Tyr70 in (F) corresponds to the structure of a conformational intermediate. This is the first structure which demonstrates that the slow conversion of DNA substrates by RIPs can be trapped during crystallization.
Models, Molecular, Guanine, Adenine, Ribose, Molecular Sequence Data, Crystallography, X-Ray, Protein Structure, Secondary, Hydrobromic Acid, Rats, RNA, Bacterial, Adenosine Triphosphate, Deoxyadenine Nucleotides, RNA, Ribosomal, Catalytic Domain, Animals, Momordica, Amino Acid Sequence, Conserved Sequence, Plant Proteins, Protein Binding
Models, Molecular, Guanine, Adenine, Ribose, Molecular Sequence Data, Crystallography, X-Ray, Protein Structure, Secondary, Hydrobromic Acid, Rats, RNA, Bacterial, Adenosine Triphosphate, Deoxyadenine Nucleotides, RNA, Ribosomal, Catalytic Domain, Animals, Momordica, Amino Acid Sequence, Conserved Sequence, Plant Proteins, Protein Binding
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