Sex-determining region Y-box 2 and GA-binding proteins regulate the transcription of liver receptor homolog-1 in early embryonic cells
Copyright policy )Sex-determining region Y-box 2 and GA-binding proteins regulate the transcription of liver receptor homolog-1 in early embryonic cells
Pluripotent stem cells maintain their pluripotency and undifferentiated status through a network of transcription factors. Liver receptor homolog-1 (Lrh-1) is one of these, and regulates the expression of other important transcription factors such as Oct-3/4 and Nanog. In early embryo and embryonic stem (ES) cells, Lrh-1 is transcribed using a unique promoter. In this study, we investigated the transcriptional regulation of embryonic Lrh-1 using ES and embryonal carcinoma F9 cells. Reporter assays, electrophoretic mobility shift assays, and chromatin immunoprecipitation assays demonstrated that Sox2 and Gabp proteins bind to the promoter region of embryonic Lrh-1, and are necessary for its activation. The Sox2 site showed strong promoter activity and affinity for protein binding. Upon differentiation into the parietal endoderm by retinoic acid and cAMP, Lrh-1 promoter activity and transcripts were markedly reduced within 24 h. At the same time, Sox2 and Gabp binding to the promoter region of Lrh-1 were decreased, followed by a reduction of their expression. These results indicate that embryonic Lrh-1 expression is regulated by both Sox2 and Gabp. Our study presents new insights into the transcription factor network of pluripotent stem cells.
Pluripotent Stem Cells, Base Sequence, Transcription, Genetic, SOXB1 Transcription Factors, Receptors, Cytoplasmic and Nuclear, Transfection, GA-Binding Protein Transcription Factor, DNA-Binding Proteins, Mice, Gene Expression Regulation, Cell Line, Tumor, Animals, Promoter Regions, Genetic, Embryonic Stem Cells, Protein Binding
Pluripotent Stem Cells, Base Sequence, Transcription, Genetic, SOXB1 Transcription Factors, Receptors, Cytoplasmic and Nuclear, Transfection, GA-Binding Protein Transcription Factor, DNA-Binding Proteins, Mice, Gene Expression Regulation, Cell Line, Tumor, Animals, Promoter Regions, Genetic, Embryonic Stem Cells, Protein Binding
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