Transcriptional regulation of the growth-regulated oncogene α gene by early growth response protein-1 in response to tumor necrosis factor α stimulation
Copyright policy )Transcriptional regulation of the growth-regulated oncogene α gene by early growth response protein-1 in response to tumor necrosis factor α stimulation
Growth-regulated oncogene α (GROα) plays an important role in a wide range of normal and pathological conditions, including inflammation, angiogenesis, wound healing, tumor invasion, and metastasis. Egr-1 is a member of the zinc-finger transcription factor family induced by diverse stimuli, including TNFα. However, the role of Egr-1 in GROα expression was previously unknown. This study shows that Egr-1 directly binds to the GROα promoter and transactivates the GROα gene. Silencing of Egr-1 by expression of Egr-1 siRNA abrogated TNFα-induced GROα transcription. We also found that Egr-1 mediates ERK and JNK MAPK-dependent GROα transcription upon TNFα stimulation. Our findings suggest that Egr-1 may play an important role in tumor development through transactivation of the GROα gene in response to TNFα within the tumor microenvironment.
- Konkuk University Korea (Republic of)
Binding Sites, Base Sequence, MAP Kinase Signaling System, Chemokine CXCL1, Blotting, Western, Molecular Sequence Data, Electrophoretic Mobility Shift Assay, Blotting, Northern, Real-Time Polymerase Chain Reaction, Gene Expression Regulation, Cell Movement, Mutation, Mutagenesis, Site-Directed, Humans, RNA, Messenger, Phosphorylation, RNA, Small Interfering, Promoter Regions, Genetic, Early Growth Response Protein 1, HeLa Cells
Binding Sites, Base Sequence, MAP Kinase Signaling System, Chemokine CXCL1, Blotting, Western, Molecular Sequence Data, Electrophoretic Mobility Shift Assay, Blotting, Northern, Real-Time Polymerase Chain Reaction, Gene Expression Regulation, Cell Movement, Mutation, Mutagenesis, Site-Directed, Humans, RNA, Messenger, Phosphorylation, RNA, Small Interfering, Promoter Regions, Genetic, Early Growth Response Protein 1, HeLa Cells
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