Insulin-like Growth Factor-I Gene and Insulin-like Growth Factor Binding Protein-3 Polymorphism in Patients with Thyroid Dysfunction
Copyright policy )Insulin-like Growth Factor-I Gene and Insulin-like Growth Factor Binding Protein-3 Polymorphism in Patients with Thyroid Dysfunction
Thyroid hormones have important roles in normal growth and skeletal muscle development. IGF-I is one of the most important growth factors and is needed for the proliferation and development of thyroid cells. It stimulates fibroblasts, follicular and endothelia cells in thyroid gland. It has been shown that thyroid hormones play an important role in the regulation of IGF-I and IGFBP-3. In this study we proposed that IGF-I (CA)(19) and IGFBP-3-202 A/C gene polymorphism may affect thyroid functions. For this purpose, frequency of IGF-I (CA)(19) and IGFBP-3-202 A/C gene polymorphism in hypo- and hyperthyroid patients and possible role of these polymorphism in thyroid functions were investigated.This study was performed on 37 volunteer hyperthyroid and 76 hypothyroid patients as well as with 50 healthy subjects as controls. DNA isolation was applied in peripheral blood samples obtained from patients and controls. Required areas were amplified with PCR by using proper primers belonging to these gene areas from the isolated DNA samples. The products were evaluated with visualization by UV gel documentation system.Frequency of IGF-I (CA)(19) gene polymorphism among hypothyroidism patients, hyperthyroidism patients and controls were statistically significant (chi(2) = 11.55, df = 4, p = 0.021). Genotypic variations between hyper- and hypothyroid patients were significant (chi(2) = 11.39, df = 2, p = 0.003), whereas there was no difference in IGF-I (CA)(19) gene polymorphism between the patients and controls. Differences in the IGFBP-3-202 A/C gene polymorphism between controls and hypo- as well as hyperthyroid patients were not significant. But IGFBP-3-202 A/C gene polymorphism genotype frequencies showed a significant difference between hypo- and hyperthyroid patients (chi(2) = 6.24, df = 2, p = 0.044).These findings suggests that IGF-I (CA)(19) and IGFBP-3-202 A/C gene polymorphisms may be a risk factor for hypothyroidism.
- Pamukkale University Turkey
Base Sequence; Case-Control Studies; DNA Primers; Humans; Insulin-Like Growth Factor Binding Protein 3/*genetics; Insulin-Like Growth Factor I/*genetics; Polymerase Chain Reaction; *Polymorphism, Genetic; Thyroid Diseases/*genetics, polymerase chain reaction, 610, somatomedin binding protein 3, gene frequency, Hyperthyroidism, Polymerase Chain Reaction, Genetic, Hypothyroidism, 616, genetic variability, hyperthyroidism, Humans, controlled study, thyroid disease, human, Polymorphism, Insulin-Like Growth Factor I, DNA Primers, Insulin like growth factor-I (IGF-I), Polymorphism, Genetic, Base Sequence, article, DNA, somatomedin C, DNA isolation, major clinical study, Thyroid Diseases, Insulin-Like Growth Factor Binding Protein 3, DNA polymorphism, Case-Control Studies, hypothyroidism, Insulin-like growth factor binding protein-3 (IGFBP-3)
Base Sequence; Case-Control Studies; DNA Primers; Humans; Insulin-Like Growth Factor Binding Protein 3/*genetics; Insulin-Like Growth Factor I/*genetics; Polymerase Chain Reaction; *Polymorphism, Genetic; Thyroid Diseases/*genetics, polymerase chain reaction, 610, somatomedin binding protein 3, gene frequency, Hyperthyroidism, Polymerase Chain Reaction, Genetic, Hypothyroidism, 616, genetic variability, hyperthyroidism, Humans, controlled study, thyroid disease, human, Polymorphism, Insulin-Like Growth Factor I, DNA Primers, Insulin like growth factor-I (IGF-I), Polymorphism, Genetic, Base Sequence, article, DNA, somatomedin C, DNA isolation, major clinical study, Thyroid Diseases, Insulin-Like Growth Factor Binding Protein 3, DNA polymorphism, Case-Control Studies, hypothyroidism, Insulin-like growth factor binding protein-3 (IGFBP-3)
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).14 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!