Abstract The spatter and the molten pool behavior, which were the important phenomena concerned with the welding quality, were observed and studied by using the high-speed camera and the X-ray transmission imaging system during laser welding under different welding parameters. The formation mechanism of spatter and the corresponding relationships between the spatter and molten pool behavior were investigated. The increase of laser power could cause more intense evaporation and lead to more spatter. When the focal position of laser beam was changed, different forms of spatter were generated, as well as the flow trends of molten metal on the front keyhole wall and at the rear molten pool were changed. The results revealed that the behavior of molten pool, which could be affected by the absorbed energy distribution in the keyhole, was the key factor to determine the spatter formation during laser welding. The relatively sound weld seam could be obtained during laser welding with the focal position located inside the metal.