AbstractA new flavonol glycoside, namely quercetin 3-O-[α-d-apiofuranosyl(1–5)-β-d-apiofuranosyl(1–2)]-α-l-rhamnopyranosyl(1–6)-β-d-glucopyranoside (1), was isolated from glandless cotton seeds together with the known compounds quercetin 3-O-α-l-rhamnopyranosyl(1–2)-[α-l-rhamnopyranosyl(1–6)]-β-d-glucopyranoside (manghaslin, 2), kaempferol 3-O-β-d-apiofruranosyl(1–2)-β-d-glucopyranoside (3) and kaempferol 3-O-α-l-rhamnopyranosyl(1–6)-β-d-glucopyranoside (4). It is interesting that the tetrasaccharide fragment of 1 contained both a β-apiosyl and an unusual α-apiosyl group.