In this paper, a novel electrochemiluminescence (ECL) imaging sensor array was developed for determination of hydrogen peroxide (H2O2), which was based on Cu/Zn alloy galvanic cell generated ECL. In alkaline solution, Cu/Zn galvanic cell was formed because of corrosion effect, the galvanic cell could supply stable potential for ECL generation of luminol, and the weak ECL emission could be enhanced by H(2)O(2). The galvanic cell sensor array was designed by putting Cu/Zn alloy in 96-well microtiter plates separately. The relative ECL intensity was proportional with the concentration of hydrogen peroxide in the range of 1.0 x 10(-6) to 1.0 x 10(-4) mol l(-1) and the detection limit was 3.0 x 10(-7) mol l(-1) (3sigma), the relative standard deviation (R.S.D.) for 11 parallel measurements of 1.0 x 10(-5)mol l(-1) H2O2 was 4.0%.