A capillary electrophoretic assay for acetyl coenzyme A carboxylase
Copyright policy )A capillary electrophoretic assay for acetyl coenzyme A carboxylase
A simple off-column capillary electrophoretic (CE) assay for measuring acetyl coenzyme A carboxylase holoenzyme (holo-ACC) activity and inhibition was developed. The two reactions catalyzed by the holo-ACC components, biotin carboxylase (BC) and carboxyltransferase (CT), were simultaneously monitored in this assay. Acetyl coenzyme A (CoA), malonyl-CoA, adenosine triphosphate (ATP), and adenosine diphosphate (ADP) were separated by capillary electrophoresis, and the depletion of ATP and acetyl-CoA as well as the production of ADP and malonyl-CoA were monitored. Inhibition of holo-ACC by the BC inhibitor, 2-amino-N,N-dibenzyloxazole-5-carboxamide, and the carboxyltransferase inhibitor, andrimid, was confirmed using this assay. A previously reported off-column CE assay for only the CT component of ACC was optimized, and an off-column CE assay for the BC component of ACC also was developed.
- Louisiana State University United States
Electrophoresis, Capillary, Adenosine Triphosphate, Acetyl Coenzyme A, Carboxyl and Carbamoyl Transferases, Biocatalysis, Escherichia coli, Carbon-Nitrogen Ligases, Enzyme Inhibitors, Holoenzymes, Acetyl-CoA Carboxylase, Enzyme Assays
Electrophoresis, Capillary, Adenosine Triphosphate, Acetyl Coenzyme A, Carboxyl and Carbamoyl Transferases, Biocatalysis, Escherichia coli, Carbon-Nitrogen Ligases, Enzyme Inhibitors, Holoenzymes, Acetyl-CoA Carboxylase, Enzyme Assays
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).8 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!