Proteins isolated with TRIzol are compatible with two-dimensional electrophoresis and mass spectrometry analyses
Copyright policy )Proteins isolated with TRIzol are compatible with two-dimensional electrophoresis and mass spectrometry analyses
TRIzol is used for RNA isolation but also permits protein recovery. We investigated whether proteins prepared with TRIzol were suitable for two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization mass spectrometry. Proteins from TRIzol-treated SH-SY5Y cells produced 2-DE spot patterns similar to those from an equivalent untreated sample. Subsequent identification of TRIzol-treated proteins using peptide mass fingerprinting was successful. TRIzol exposure altered neither the mass of myoglobin extracted from sodium dodecyl sulfate (SDS) gels nor the masses of myoglobin peptides produced by in-gel trypsin digestion. These findings suggest that proteins isolated with TRIzol remain amenable to proteomic analyses.
- Massey University New Zealand
- Institute of Environmental Science and Research New Zealand
Proteomics, Molecular Sequence Data, 0601 Biochemistry and Cell Biology, Guanidines, Peptide Mapping, Phenols, Cell Line, Tumor, 0399 Other Chemical Sciences, Animals, Humans, Electrophoresis, Gel, Two-Dimensional, Trypsin, Peptide mass fingerprint, Protein extraction, Amino Acid Sequence, Horses, TRIzol, Mass spectrometry, Myoglobin, Proteins, 2-DE, Neoplasm Proteins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, 0301 Analytical Chemistry
Proteomics, Molecular Sequence Data, 0601 Biochemistry and Cell Biology, Guanidines, Peptide Mapping, Phenols, Cell Line, Tumor, 0399 Other Chemical Sciences, Animals, Humans, Electrophoresis, Gel, Two-Dimensional, Trypsin, Peptide mass fingerprint, Protein extraction, Amino Acid Sequence, Horses, TRIzol, Mass spectrometry, Myoglobin, Proteins, 2-DE, Neoplasm Proteins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, 0301 Analytical Chemistry
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